Sentence examples for cleaved out from inspiring English sources

Here, portions of the original translation product have to be cleaved out (inteins) and others joined together (exteins) before yielding a functional protein.

The entire gene was cleaved out using HindIII and blunt-end ligated into the piggyBac-containing plasmid pIR-eGFP [8], which had been digested with XhoI and NotI.

The His-tagged ORF of recB gene was then cleaved out from pETB as (XbaI-SacI) fragment and ligated into pGL10 to generate pGB.

The integrity of the inserts was confirmed by sequencing and cleaved out using BamHI and ligated into plasmid pAHIR1-β-gal (Campbell, 1996} cleaved with BglII, resulting in the plasmid pMGD72 (PPARδ).

Similarly, for the construction of pGECB that produced dimeric RecBCEc protein, 11.7 kbp DNA containing the recC-ptr-recB region of E. coli was cleaved out from the plasmid pAMP3 [54] as BamH1 fragment and then cloned into the BamH1 site of pGL10.

The 35S-GFP cassette was cleaved out with EcoRI and HindIII, and exposed to the action of several methylation-sensitive enzymes (Bsu15I, Eco72I and Eco47I).

The inability to cleave out the PMEI domain (pro-region) would presumably prevent the export of the PMEs to the cell wall, according to Wolf et al.[ 8], who showed that unprocessed Type-1PMEs are retained in the Golgi apparatus.

Inteins self-catalytically cleave out of precursor proteins while ligating the surrounding extein fragments with a native peptide bond.

Another possible enzyme to cleave out the small RNAs (especially for the product with the same size to the siRNA and miRNA) is an RNase III enzyme, such as Dicer, which cleaves dsRNAs and produce 5'-end phosphorylated RNAs.

When the aim is to obtain a specific peptide in a sample for detection or quantification with MS, the digestion process needs to include an enzyme which cleaves out this peptide either directly from a protein sequence or in a subsequent digestion step in a multi-enzyme workflow.

He was just very lovely and gave me encouragement, but I think you have to cleave it out yourself.