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The templates were coupled by linkers to cellulose membranes of microplate format, which could be cleaved for control of intermediate and final products.
It seemed to be "violating the dogma" established with HIV and flu that the viral surface protein needs to be cleaved for the virus to enter the cell.
APPL is a transmembrane protein that is cleaved for secretion [4].
These derivates were not intended for the design of a future library, as they could not be cleaved for evaluation, but were used only to test this hypothesis.
MBP, Golli-MBP BG21 and Golli-MBP J37 (5 µM each) were cleaved for 1 h at 37°C by MT6-MMP (0.05 µM; an enzyme-substrate ratio of 1∶100).
This may reflect insufficient activation of MMP-2, as the precursor protein must be cleaved for enzymatic function [ 52].
Similar(52)
It is not possible to predict where in a peptide cathepsin B (C01.060) will cleave, for example, despite its known preferences for a hydrophobic residue in the S2 pocket and arginine in S1 (18).
Murine KS (for anti-idiotype antibodies), huFc-IL2 (for anti-Fc-IL2 antibodies) and IL2 cleaved from huKS-IL2 (for anti-IL2 antibodies) were used as capture reagents.
PHM and PAL activities were significantly correlated (p < 0.05), as would be expected for enzymes cleaved from the same bifunctional precursor.
Activated caspase-3 then cleaved XIAP for proteasomal degradation, thereby initiating a positive amplification loop causing enhanced apoptosis.
Immunostaining of γH2AX (marker for double strand DNA breaks), pChk1S296 (marker of Chk1 activation), phistone H3 (marker for mitosis), cleaved caspase 3 (CC3) (marker for apoptosis), and pS6 were performed.
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