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Caspase-3 antibody CS-9665SS) and cleaved caspase-3 (Cleaved Caspase-3 CS-9661SS)) were purchased from Cell Signaling and generously provided by Dr. Jun Tsuji (NCSU).
Pro-caspase-3 (37 kDa) was cleaved into 17 kDa fragment (cleaved caspase-3) during apoptosis.
Cleaved caspase 3 flow cytometry was performed using the cleaved caspase-3 (Asp175) (5A1) rabbit monoclonal antibody (Cell Signaling, Hitchin, UK) according to the manufacturer's instructions.
b Quantitative analysis of cleaved caspase-3.
(C) Graph shows normalized level of cleaved caspase-3.
a Western blot results for cleaved caspase-3 protein.
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Cleaved caspase-3 was detected using rabbit anti-cleaved caspase-3 antibody diluted 1 1000 (Cell Signalling No. 9661).
Levels of cleaved caspase 3 were increased in the hippocampus of AD rats.
The number of cleaved caspase 3-positive germ cells per tubule was markedly increased.
The degree of co-localization of OX1R with cleaved caspase 3 was more than 90%.
Apoptosis was evaluated by counting cleaved caspase 3 positive cells (Figure 2G, H).
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