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Three slides were then probed for signs of apoptosis by immunofluorescence with antibodies against cleaved caspase 3, cleaved caspase 9, and cleaved PARP.
Above changes were related to altered cell demise (apoptosis and necroptosis), as shown by upregulated expression of cleaved caspase 9, cleaved caspase 3 and RIP1/RIP3/MLKL cascade.
Here we observed elevated levels of cleaved Caspase 8 and cleaved Caspase 3 and comparatively lower elevation of tBid (Figure 7B).
Levels of cleaved caspase 3 were increased in the hippocampus of AD rats.
The number of cleaved caspase 3-positive germ cells per tubule was markedly increased.
Apoptotic cells are revealed by cleaved caspase 3 (cCastainingining.
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Upon O. kansuensis treatment, cleaved caspase-8, cleaved caspase-9 and cleaved caspase-3 levels increased with increased toxic doses, cleaved caspase-8 and cleaved caspase-9 showed a dosed-dependent increase.
Western blotting was done with antibodies against cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 to delineate lutein's mechanism of action in the apoptotic cascade.
Pro-caspase-3 (37 kDa) was cleaved into 17 kDa fragment (cleaved caspase-3) during apoptosis.
Furthermore, sorafenib administration induced elevated protein level of cleaved caspase-9, cleaved caspase-3, and cleaved PARP dose-dependently).
b Quantitative analysis of cleaved caspase-3.
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