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To study the differences in the cleavage time between types of embryo chromosomal abnormalities and elaborate algorithm to exclude aneuploid embryos according to the likelihood to be euploid.
To study the differences in the cleavage time between chromosomally normal and abnormal embryos and to elaborate an algorithm to increase the probability of noninvasively selecting chromosomally normal embryos.
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As demonstrated in Figure 6A, each stochastic time trajectory of CEA presents a time delay between time zero, the CC release time, and the CEA activation time, which is conventionally defined as the half-maximal caspase 3 cleavage time.
Secondary endpoints include using the Embryoscope to examine embryo quality, including S2 (synchrony during second set of cleavage divisions), T5 (time between fertilisation and the five cell embryo formation) [ 14, 24], CC3 (duration of the third cell cycle) [ 24], and the appearance and disappearance of the pronuclei [ 25].
A detailed retrospective analysis of time-lapse microscopy results showed that several parameters of developmental dynamics were significantly correlated with subsequent implantation (e.g., time of first and subsequent cleavages as well as the time between cleavages).
We do not know how much effect this shrinkage has on early development during cleavages and we must, therefore, stress that the time between cleavages reported here should be taken with caution.
During culture of the eggs in vitro, the time between the earliest cleavages was roughly 24 h at 13 °C.
As absence of caspase feedbacks results in an overall slower signalling during apoptosis execution, the prolonged lag time between MOMP and substrate cleavage allows for diffusion to efficiently eliminate spatial anisotropies.
The maximum lag time between the embryos was observed to be approximately one cleavage, corresponding to a developmental time of 12 to 15 minutes [ 27].
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