Sentence examples for cleavage strain from inspiring English sources

The resulting Spc110 cleavage strain is KGY57.

All three Spc110 cleavage strain markers (SPC110-3XTEV696-HA::natMX4, GAL-TEV::URA3, and GAL2 LEU2), as well as the xΔ::KAN marker for the deletion, were selected for.

The resulting strain, KGY39, was then transformed with the SPC110-3xTEV696 cassette as described in the Spc110 cleavage strain (W303 background) section above to make strain KGY53, and correct integration of the cleavage sites was confirmed by sequencing.

This plasmid was then converted to an integrating plasmid by ligating the pRS306 [29] large AlwN1 fragment to the pKG16 large AlwN1 fragment, resulting in the pKG17 plasmid used for Spc110 cleavage strain construction.

Spc110 cleavage strains form colonies on plates under induced conditions (Figure 1C), indicating that the cells are able to assemble functional SPBs even after Spc110 cleavage.

The spindle checkpoint is required to maintain viability of Spc110 cleavage strains under induced conditions (Figure 1D), indicating that a cell cycle delay is necessary to allow for correct spindle assembly in the presence of Spc110 cleavage.

YPD HYG plates are standard YPD supplemented with 0.6 mg/ml hygromycin B. Media used in the SGA screens were made as previously described [26] except where tailored to fit the Spc110 cleavage strains as noted below.

We also found that both Novosphingobium strains encoded homologues of the NAD-dependent dehydrogenases that are essential for forming the α-ketones that undergo β-ether cleavage in strain SYK-6.

As Cas9 endonuclease is a universal component for any gRNA-mediated DNA cleavage, transgenic strains expressing Cas9 nuclease endogenously have been made in Drosophila (Kondo and Ueda, 2013; Ren et al., 2013; Sebo et al., 2013).

in vitro: The mechanochemical relationship between enzymatic cleavage and applied strain shown here could be exploited, by controlling the network strain state and introducing enzymes, to produce highly organized tissue engineering constructs which can be actively remodeled in vivo.

Moreover, spore formation of the ΔCtpB/4FA double mutant was reduced to the same level as for the 4FA variant missing all 4B cleavage sites, whereas strains either lacking CtpB or expressing 4FA were almost indistinguishable from wild-type.