Sentence examples for cleavage size from inspiring English sources
The enterokinase cleavage size is present just upstream of the cloned NS1 gene.
The Ana2 CCCD was purified from its diLipoyl fusion protein by proteolytic cleavage, size exclusion, and ion exchange chromatography.
To analyse flap cleavage size, reaction products were separated on a 20% polyacrylamide, 8 M urea, 1 × TBE gel and visualised by autoradiography.
We are thus unable to provide some ground at the molecular level to such a variation in cleavage size nor to discriminate between the two NHEJ processes possibly involved in indel slippage.
As before, full-length synaptotagmin 1 was expressed in Sf9 insect cells (Invitrogen, Grand Island, NY), purified by Ni2+-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) affinity chromatography, followed by his-tag cleavage, size exclusion chromatography, and, finally, ion exchange chromatography.
Proteins were expressed in E. coli BL21 Rosetta and purified using standard methods via NiNTA (Qiagen, Hilden, Germany) chromatography, proteolytic tag cleavage, size-exclusion chromatography and ion-exchange chromatography.
Cleavage sizes should be reduced by one nucleotide in the case of NHEJ with direct fill-in of complementary ends (Fig. 5 c ). Chemicals (toxic agents) and radiations (UV, IR, X-rays) are natural sources of DSB, although the biochemical processes involved are not well understood.
As they say down at Dolce & Gabbana, she adorns pulse points, although even she had to brake in shock beside a billboard in Los Angeles, brought up short by what she called "my cleavage the size of a brontosaurus".
Following Ni-NTA chromatography, TEV cleavage and size exclusion, proteins were concentrated to 10 15 mg/ml in 10 mM NaP04 (pH 7.5), 150 mM NaCl.
Purification using glutathione beads followed by GST cleavage and size exclusion chromatography yielded 4 mg of pure AfAGM1 per litre of bacterial culture.
The fusion protein was expressed in E. coli B834 (DE3) as an N-terminally His-tagged fusion, and purified via NiNTA chromatography, proteolytic tag cleavage and size exclusion chromatography.
