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A too high concentration (400 μM) significantly (p < 0.05) decreased the cleavage rate and blastocyst yield.
Thus, detection of glucose could be realized by monitoring the cleavage rate of PLDz.
Good and fair quality oocytes produced a higher cleavage rate (P<0.01) than poor quality oocytes.
Experimentally, alteration of downstream phosphate oxygen either by 2′-5′ sugar linkage or stereo-specific thio-substitution of phosphate oxygen drastically reduced cleavage rate.
Although the cleavage rate in pH 7.4 phosphate buffered saline (PBS) at 37 °C was slow (t1/2 = 35.7 h), a rapid cleavage rate was observed in 2% NaOH aq (t1/2 = 1.5 min).
For 10 of 15 droplet bulls, <10% of ova were cleaved whereas cleavage rate was >15% for all control bulls.
Cleavage rate was recorded at 48 h postinsemination, and blastocyst development was recorded on Days 6 to 9.
Uncleaved oocytes were cultured for an additional 24 h at this time, cleavage rate was similar between breeds.
The design was based on proteochemometric models for Michaelis (Km) and cleavage rate constants (kcat) of protease substrates.
Further, we found that the off-target cleavage rate is reduced using Cas9 protein when compared to plasmid DNA transfection.
IGF-I failed to improve cleavage rate, as well as blastocyst production, when added during in vitro culture (IVC).
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