Sentence examples for cleavage quantification from inspiring English sources
In short, each cell line DNA sample was digested with HpaII and EcoR1 or MspI and EcoR1 followed by cleavage quantification using Pyrosequencing in a PSQ96 MA system (Biotage AB, Uppsala, Sweden).
In addition to PARP cleavage assay, quantification of apoptosis was also evaluated by checking Caspase-3/7 activations.
Because the number of these sequences is known and very low in samples comprised by an identifiable number of cells, such as early embryos that have undergone few cleavages, their precise quantification provided an optimal internal control to demonstrate the strength of this novel technique.
The method we employed was based upon our previous sequence-specific rRNA cleavage method that allows quantification of rRNA of target groups so that the populations reflecting in situ activity could be determined.
Quantification of cleavage bands was performed using ImageLab software (Bio-Rad, Hercules, CA).
When including proteolytic cleavage products in the quantification, the total detected amount found in the cells was around 1200 mg/L.
In addition, QPCR allowed an immediate quantification of the cleavage efficiency that could be useful for evaluation of the DNAzyme activity.
The N-terminal SMN antibody, followed by a secondary antibody conjugated to Cy3 (GE Healthcare, 1∶4000) was used for quantification of calpain cleavage by fluorometry using unsaturated digital scans performed on a Typhoon Trio+ Variable Mode Imager (GE Healthcare).
Quantification of substrate cleavage was assessed using a fluorometric reader (355 nm excitation, 510 nm emission).
For quantification of DNA cleavage, the DNA fragmentation assay was performed using a diphenylamine colorimetric assay [ 31, 32].
In contrast, Li/LdSIDER2A 81 Rz activity was reduced and LmSIDER2A 81 Rz increased when the 20 nt-long wild type upstream sequence was assayed (see Figure 3C for activity and 3D for quantification of the cleavage products of the constructs shown in Figure 3A).
