In this study, the Q-RT-PCR system is based on the detection of the fluorescent activity and quantification of the TaqMan® probe, which undergoes cleavage in proportion to the amount of PCR product formed [ 5, 6].
A proportion of cleavage events were attributed to the cleavage of substrates containing two or more nicks, and it is unclear whether the transformed plasmid-based substrate accurately mimics an intact HJ substrate.
Maturation of ovine oocytes in coculture with bovine granulosa cells not exposed to NH4Cl (Experiment 3) increased egg cleavage rate and the proportion of cleaved eggs which developed to the blastocyst stage.
In contrast to the selective reduction of AICD and increase in CTFs seen following the knockdown of any one of the four γ-secretase components, both ubqn loss-of-function and ubqn overexpression modify the levels of APP and its cleavage fragments largely in proportion.
Hence, NE plus palmitate induce Opa1 cleavage, thereby increasing the proportion of fusion-deficient short forms.
Moreover, the relative distance of a pair of sites to an uncapped end also alters the relative proportion of cleavage at each site in a manner consistent with sliding and end-dependent dissociation (K. van Aelst, personal communication).
No obvious apoptosis resulted from BIS silencing as assessed by subG1 proportion, PARP cleavage and Annexin V staining, which was distinguished from the typical features of apoptosis induced by doxorubicin.
Because the two drug molecules appear to bind independently, a high proportion of cleavage complexes established at clinically relevant (i.e., subsaturating) concentrations of etoposide contain only one cleaved DNA strand.
Activities of PARP and caspase-3 were measured as the proportion of cleavage band intensity to the total bands and were calculated as follows: % PARP or caspase-3 = 100% × Tc/Tt, where Tc is the intensity value of the cleavage bands and Tt is the intensity value of total bands.
However, the configuration of mixing significantly influenced the proportion of attrition and cleavage mechanisms.
