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We therefore measured the extent of glmS ribozyme cleavage over time at varying concentrations of GlcN6P (Figures 2b and S3).
Collagen fibrils become resistant to cleavage over time.
The extent of cleavage over time was visualized on Coomassie-stained 15% SDS PAGE gels.
We took advantage of ZP2 sensitivity to protease-mediated cleavage over time to determine if protease activity within the cKO oviducts was increased.
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Cleavage yields over time refer to the left y-axis (% cleavage yield); other data points refer to the right y-axis (F/Fo, fluorescence enhancement).
DOI: http://dx.doi.org/10.7554/eLife.07545.018 10.7554/eLiFigure4 figureigure 4—figure supplement 1. Human standard- and immuno-proteasomes vary their cleavage activities over time.
In line with the augmented caspase activity, we observed an increase in caspase cleavage products over time in TRAIL-stimulated FAT1 knockdown cells.
"People change over time.
Furthermore, the alloc side group and its photopolymerization products did not create any significant steric barriers or noticeable changes to the MMP-dependent cleavage over the time periods investigated.
Therefore substrate depletion, product re-entry or further cleavage cannot explain variation in cleavage site usage over time.
As a first step we are interested in whether our fully parameterised model can explain the altered cleavage site usage over time.
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