Some omega peptidases (peptidases which do not cleave normal peptide bonds but release substituted amino acids such as pyroglutamate or cleave isopeptide bonds, such as many deubiquitinating enzymes) may also be unable to accept any residue in certain positions, or it is not possible to interpret cleavages in terms of P4 P4′, for example for isopeptidases.
Emphasis is placed on the incorporation of plastic strain effects into the probabilistic framework by approaching the strong influence of constraint variations on (macroscopic) cleavage fracture toughness in terms of the number of eligible Griffith-like microcracks which effectively control unstable crack propagation by cleavage.
This paper provides an overview of recent progress in probabilistic modeling of cleavage fracture phrased in terms of a local approach to fracture (LAF) and the Weibull stress concept.
The extent of DNA cleavage was assessed in terms of intact sites in methylation-insensitive digestions.
Both mutants show a slight (2.4-fold for K98A) or strong (140-fold for D308A) decrease in turnover (kcat) for DHUG cleavage which is compensated, in terms of efficiency, by lower KM values (Table 1), particularly for the D308A mutant (34-fold).
Other intein systems, such as the Ssp DnaE intein, the Mtu RecA intein, and the Sce VMA intein, exhibit tight coordination between the N- and C-terminal cleavage events that is explained in terms of a conformational change during N-terminal cleavage, which optimally positions residues for C-terminal cleavage.
In terms of cleavage activity, the four mutant Tld proteins show reduced cleavage of Sog, especially of the 33 kDa fragment, consistent with their reduced ability to bind Sog and the requirement for Collagen IV for Tsg enhancement of processing at the 33 kDa site.
By examining the conservation of cleavage sites in protein substrates in terms of peptidase substrate binding sites, it is clear that there are a number of cleavages where atypical replacements occur.
In fact, an emerging feature of current times is the onset on a new cleavage that can be characterised in terms of two competing conceptions of the nation, an open versus a closed conception of the national community.
The regioselectivity of such methoxylation and C N cleavage can be explained mainly in terms of the α-CH kinetic acidities of the cation radicals formed by one-electron oxidation of the amines.
