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Advances with tailoring HEs to achieve target-specific cleavage have also been significant, but the coupled sequence-binding and enzymatic cleavage functions restrict the ease with which HEs may be engineered.
The msh2 missense alleles used in this study were derived from plasmids originally generated by Studamire et al. [61] to study the separation of the mismatch repair and non-homologous tail cleavage functions of Msh2.
G d And G s are the Dicer cleavage functions for dsRNA and ssRNA respectively.
We propose that the cytoplasmic 20S pre-rRNA cleavage functions as one of the checkpoints that prevent incompletely assembled, pre-40S subunits from entering translation.
Although concomitant loss of WRN and MUS81 was shown to limit DSB formation in response to HU, it induced cell death, indicating that deliberate RF cleavage functions as a survival mechanism in this setting.
As well as nuclear rRNA and mitochondrial primer cleavage functions, in S. cerevisiae at least, it has an additional function of promoting cell cycle progressing by cleaving CLB2 mRNA in its 5' UTR region at the end of mitosis [ 52, 53].
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Here an exonuclease III (Exo III -assisted cascade autocatalytIII -assistedamplificascade(Exo-Cautocatalytic is precyclingor lamplificationmiluminescent (CL) detection of platelet-derived growth factor BB (PDGF-BB) by taking advantage of both recognition property of aptamer and cleavage function of Exo III.
Search of the tomato degradome confirmed the cleavage function of miR6024 and also suggest the cleavage function of tasiRNAs triggered by miRNAs (see Results section).
k ri is the saturation constant of the siRISC cleavage function.
When single and double-stranded RNA cleavage by Dicer are combined, the Dicer cleavage function is saturated for both single- and double-strand RNA.
Another strategy for disrupting the cleavage function of AGO2 is to remove the potential for base interactions entirely by using abasic substitutions.
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