We show that these toxins exhibit NS3 cleavage dependent increase in enzymatic activity upon NS3 protease cleavage in vitro.
Galectin-3 cleavage, dependent on MMP9 upregulation, facilitated formation of a galectin-3/MUC1/EGFR complex.
As well as meeting the requirement for discrimination of a single base point mutation, the method described provides a simple mechanism to vary the rate of cleavage dependent on the orientation of the mismatch relative to the mutation site and requires only transient binding of the oligonucleotide to the template while the S1 nuclease is present to cleave the substrates.
However, Cu2+-induced catalytic reaction disturbs the graphene DNAzyme conformation, which will produce internal DNA cleavage-dependent effect.
Gilman MS, Moin SM, Mas V, Chen M, Patel NK, Kramer K, Zhu Q, Kabeche SC, Kumar A, Palomo C, Beaumont T, Baxa U, Ulbrandt ND, Melero JA, Graham BS, McLellan JS, "Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein," PLoS Pathog 11, e1005035 (2015).
Subsequent cleavage-dependent activation of effector caspases leads to cellular demise.
Cytokine maturation is cleavage-dependent, and it is mediated by non-apoptotic caspases such as caspase-1 [ 136].
We first investigated whether MESA constructs were expressed, localized to the cell surface, and signaled in a cleavage-dependent fashion.
In the experiments testing the thrombin-cleavage-dependent effects of protein S, I am not convinced the protein S has been fully processed.
Figure 5a shows that vector but not PRELI transfectants undergo STS-induced, cleavage-dependent activation of caspase-9, 8 and 3.
These results are consistent with the hypothesis that duplexes functioning through a non-cleavage-dependent miRNA-like mechanism are better able to discriminate between normal and extended CAG repeat targets.
