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To investigate the potential effects of Beclin 1 mutation against caspase cleavage, cells were transfected with the constructed EGFP-Beclin 1-WT, EGFP-Beclin 1-D121/124A, EGFP-Beclin 1-D146/149andnd EGFP-Beclin 1-D121/124/146/149A plandids, and the distribution of fluorescent protein was monitored using a live cell station.
To investigate the potential effects of Beclin 1 mutation against caspase cleavage, cells were transfected with EGFP-Beclin 1-WT, EGFP-Beclin 1-D121/124A, EGFP-Beclin 1-D146/149A, EGFP-Beclin 1-D121/124/146/149A, pcDNA3.1-iCasper-T2A-HO1, PLVX-iCasper (146/149 WT -Puro or PLVX-iCasper (D146/149A mutant)-Puro plasmids.
In order to improve the amount of cleavage, cells were transfected with 3 µg of pBud-mem-sTEVp.
To address which protease is responsible for the intramembranous cleavage, cells were treated with γ-secretase inhibitor DAPT.
To study caspase 3 activation and PARP cleavage, cells were treated with rhTRAIL, anti-CD95, cycloheximide, or combinations of cycloheximide with rhTRAIL or anti-CD95 for 24 h.
To measure caspase-3 cleavage, cells were harvested and incubated with the FITC-DEVD-FMK (BioVision, Milpitas, CA, USA) in PBS in a 37°C incubator with 5% CO2 for 30 min.
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miR-21, a mature miRNA, has been implicated in different cell processes such as cell proliferation, mitochondrial damage, cell growth, chemosensitivity, cell cycle, genome instability, response to stress, mRNA cleavage, cell invasion and translation.
At the beginning of cleavage, cell divisions tend to occur at the same time in all blastomeres, and the number of cells is doubled at each division.
Carbamate and ester cleavage Drug abuse treatment, biocatalysis [117, 118] Amidase AmpD (Citrobacter freundii) Amide cleavage, cell wall recycling Diagnostics [119 121].
Embryos were examined for cleavage (cell number) and grade, which includes cytoplastmic fragmentation.
Their sisters 1abc122 divide 860 905 minutes after first cleavage (cell cycles≈340 360 min) (Figure 19).
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