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In consensus, transposon-related initial targets have considerably less amount of supported cleavage cases compared to non-transposon or expressed proteins (Figure 5).
Similar(59)
We observed cleavage in case of Cdc5, Cdc14, Cdc48, Cyr1, and Mcm1 (see Additional file 1 Figure S2).
With substrates containing either linkage, the HDV ribozyme generated 2',3'-cyclic phosphate and 5'-hydroxyl groups suggesting that the mechanisms of cleavage in both cases were by a nucleophilic attack on the phosphorus center by the adjacent hydroxyl group.
In some cases, cleavage of the wild-type receptor occurs at the cell surface [29].
In all cases cleavage is accompanied by halogenation.
In rarely demonstrated cases, cleavage triggered by near-perfect complementarity [ 25] has been observed.
In both cases cleavage was high – indeed higher (almost 100%) in vivo.
In certain cases, cleavage assays were performed after heterocyclization by adding protease to the ThcD/ThcA reaction mixture while maintaining the same final concentration as detailed above.
In this case, cleavage of cohesin solely in the vicinity of kinetochores should have little effect.
One missed cleavage site in case of incomplete trypsin hydrolysis was allowed.
Of note, there was no protection from cleavage in the case of Bid pretreatment with lysate from vector-transfected cells.
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