During death receptor-induced apoptosis, Bid is activated through cleavage by caspase-8 or caspase-10, generating a truncated form, t-Bid, [18], [19].
Plg is activated at its site of action by proteolytical cleavage by one of three proteases, urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (tPA) [7], [8,] or the newly identified Plg activator, plasma kallikrein [9].
Plasmin, the main fibrinolytic enzyme in the body, is formed from plasminogen (Plg) by proteolytic cleavage by one of three Plg activators, the urokinase type (uPA), tissue type (tPA) [15] or plasma kallikrein [16].
PCR without digestion was also used to screen for larger indel mutations and long-range deletions resulting from simultaneous cleavage by two TALEN pairs.
The inactive precursors of SREBPs are adhered to the endoplasmic reticulum (ER) and their activation requires cleavage by two proteases in the Golgi apparatus [ 4].
Simultaneous cleavage by two TALEN pairs targeting different sites of the same locus or chromosome was shown to induce long-range deletions in different species (Gupta et al. 2013).
The transfer of sucrose to the sink organs often requires its cleavage by two key enzymes: invertase (Inv), which hydrolyzes sucrose into glucose and fructose, and sucrose synthase (Sus), which is capable of catalyzing a reversible reaction but preferring to convert sucrose and UDP into fructose and UDP-glucose [ 2- 4].
DOI: http://dx.doi.org/10.7554/eLife.01861.014 An alternative way to distinguish between cleavages by a single H4 and cleavages by two H4s across a dyad is to measure distances between the left ends of fragments, W–W', and the right ends, C–C'.
The fractional cleavage by these three proteins (1 µg) at saturating concentration of poly(A) (3 nM) is shown in Fig. 6C.
