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(a) Cleavage analysis of I-CreI_D75N_target (left panel) vs I-CreI_D75N_target-null (right panel) and (b) I-CreI_3115_target (left panel) vs I-CreI_3115_target-null I-CreI_3115_target-null I-CreI_3115_target-null
Apoptosis progress was evaluated by determining cell morphology changes and caspase-3/-7 activation by PARP cleavage analysis.
For instance, constraints using structural inference of native RNAs by high throughput contact mapping, such as the multiplexed hydroxyl radical (–OH) cleavage analysis (MOHCA), improve the FARNA's prediction (Das et al. 2008).
We performed further in vitro cleavage analysis using Dβ1 23RSS carrying mutations in the spacer.
Here, using GAMAREP and AICDREP, in conjunction with in vivo cleavage analysis, we have identified factors that regulate APP.
Based on the results of mutants synapse and cleavage analysis, we have found more functional conserved residuals in the N-terminus of phiC31 integrase.
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(a) In vitro cleavage titration analysis for I-CreI_D75N_target and I-CreI_3115_target using linearized plasmids containing the cleavable target sequences.
HFE gene mutations were determined in buffy-coat DNA by PCR amplification using published primers (18), followed by restriction enzyme cleavage and analysis on a 3% agarose gel.
Considering the mitotic error rate during the cleavage stage, analysis of single blastomeres will not provide insight in the genomic constitution of the other cells, nor in the developmental potential of the embryo.
The most commonly used approaches are docking simulations (see, e.g., [ 16– 19]) and protein cleavage site analysis (see, e.g., [ 8, 12, 13]) based on Chou's distorted key theory [ 14].
It is possible to deduce their relevance and cleavage by analysis of redundant EST sequences and of genomic PAS [ 17]. EST cDNA libraries are produced using priming to the poly-A tail of the transcript and therefore ESTs will generally not represent the totality of the transcript but its 3' end, up to around 800 nt.
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