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The values were calculated by the ratio between the cleavage amount and the total radioactivity of each lane, subtracting the control background.
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The cleavage product amount was calculated as the percentage of total input mRNA.
We thus propose that during calcium-stress-induced necrosis, cleavages decrease the amount of Met receptor and generate a potential extracellular decoy fragment.
Many camisoles have adjustable straps, so you can extend the neckline of the camisole to cover up any cleavage or uncomfortable amounts of skin.
In manual mixing experiments we have found that the amount of cleavage obtained 5 sec after initiation of cleavage is identical to that obtained in 16 min, and that addition of formamide to 50% v/v immediately quenches the reaction (though the 'quenching' may be due to disruption of the transcription complex rather than quenching of the chemistry).
Group II particles were most abundant in Δ rimP and BW-25113+pU23, and overall the levels of particles belonging to Group II in each strain was consistent with the amount of cleavage observed for anti-PK-oligo-dependent RNase H cleavage.
Furthermore, the amount of cleavage fragments was significantly enhanced in RA synovial fluid compared to OA, suggesting that cleavage may correlate with the amount of synovial tissue or inflammatory signals.
(Cii ) Graph shows the amount of cleavage measured as the amount of the 50 kDa and 33 kDa fragments quantified relative to cleavage calculated for wildtype Tld in the absence of Tsg, based on three different experiments including the Western blot shown in i. Error bars show SEM, n = 3, *p < 0.005, **p < 0.001.
No changes were observed in either the total protein amounts or cleavage of NCX1, another NCX family member, in any neurodegenerative tauopathy brain.
There wasn't an unseemly amount of cleavage showing, but there it was".
It was an old story — money, gravity, the right amount of cleavage.
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