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Consistently, the caspase-3 cleavage activities were significantly activated in A549 cells from animals challenged with TRAIL plus rotenone, meanwhile, this effect was attenuated by NAC.
(c) FnCas9 target DNA cleavage activities under different conditions.
(b) FnCpf1 target cleavage activities (% indels) with or without SpdCas9 binding at proximal locations.
(b) NcCas9 target cleavage activities (% indels) with or without the assistance of SpdCas9 binding at proximal locations.
(b) CjCas9 cleavage activities on the HBB and HBD identical targets in different combinations with SpdCas9 and Sp-sgRNAs.
(d) CjCas9 cleavage activities (% indels) on the three POR targets with or without the assistance of SpdCas9.
Five multinuclear cyclotriphosphazene ligands were synthesized and tested for their cleavage activities to plasmid DNA.
FnCas9 and SpCas9 were respectively targeted to the same genomic sites in K562 cells and the cleavage activities were measured by Surveyor Nuclease S digestion assays.
(b) Cleavage activities (% indels) of FnCas9 and SpCas9 with the wild-type guide sequence (n=3, error bar shows mean±s.d.d.d
Intriguingly, the D283N APE1 mutant protein retained endoribonuclease and abasic single-stranded RNA cleavage activities, with concurrent loss of apurinic/apyrimidinic (AP) site cleavage activities on double-stranded DNA and single-stranded DNA (ssDNA).
CpMan5B is a glycoside hydrolase (GH) family 5 enzyme exhibiting both β-1,4-mannosidic and β-1,4-glucosidic cleavage activities.
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