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The moderate binding ability and unobvious DNA cleavage ability account for their low toxicity towards DNA.
The DNA cleavage ability of the complexes was monitored by gel electrophoresis using supercoiled pUC18 DNA.
Bovine and human pancreatic ribonucleases are monomers with no biological properties other than their RNA cleavage ability.
Comparative DNA oxidative cleavage ability of the complexes has been done under ultraviolet photo radiation on pUC19 DNA.
Comparative DNA oxidative cleavage ability of the complexes has been accomplished under UV photo radiation on pBR322 DNA.
Partial restoration of DNA cleavage ability was obtained by re-engineering the LAGLIDADG dimerization interface (H-DmoC).
Similar(46)
The efficacy of these derivatives to inhibit in vivo angiogenesis was evaluated using chick chorioallantoic membrane (CAM) model and their DNA cleavage abilities were evaluated after incubating with calf thymus DNA followed by gel electrophoresis.
In view of the importance of picolinic acid (PA) in preventing cell growth and arresting cell cycle, new PA based metallonucleases were designed with a view to study their DNA binding and cleavage abilities.
ADAM17 (A Disintegrin and A Metalloproteinase 17), also known as tumor necrosis factor-alpha converting enzyme (TACE) or less commonly CD156q, is a therapeutic target in multiple diseases since major contemporary pathologies like cancer, inflammatory and vascular diseases seem to be connected to its cleavage abilities [ 40].
Integrity of Scc1 might be important to mediate its stabilising function between Smc1 and Smc3, and after cleavage, its ability to connect the Smc heads might be disrupted (Gruber et al, 2003).
Transiently transfected MKK mutants were tested for expression, resistance to LT cleavage and their ability to relay exogenous signals to specific downstream MAPKs in LT-treated cells (Figure S5A).
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