Similar to the phylogenetic analyses, obvious groups like Clades A, B, and E were clearly differentiated (supplementary fig. S2, Supplementary Material online) but most other isolates showed mixed ancestry, consistent with a history of recombination.
Moreover, ∆makCB, ∆makDCB, ∆makBA, and ∆makDCBA mutants were clearly attenuated (Supplementary Fig. 1B).
While major disturbances during La Niña years tend to exhibit the opposite of these features6,7,25,27,28,29 asymmetries in rainfall anomalies are clearly evident23 (Supplementary Fig. 1b).
As the mice aged, obvious differences in tumor growth were clearly visible (Supplementary Figure 2A, available at Carcinogenesis Online).
This lack of observable density for Y527 stands in contrast to the original TEAS·FHP complex where this residue is clearly discernible (Supplementary Figure S2).
In particular, two additional β domains (R8 and R9) and the long helical domain, for which no information was included in the initial model were clearly defined (Supplementary Figure 3).
A movie of these 2D class averages from the recombinant human dynein particles allows the range of different conformations adopted by the heads to be clearly visualised (Supplementary Movie S1).
The values for gg/ gt/ tg conformations for each ring conformation clearly differ (Supplementary Tables 13 16), which corresponds to different geometries and could be used as a dictionary for these parameters.
At Day 55, in control and P301L neurons the embryonic 0N3R tau isoform was strongly expressed, in some experiments a band corresponding to 0N4R tau could be clearly seen (Supplementary Fig. 4), this would be in agreement with the immunocytochemistry data indicating 4R tau expression at Day 55.
In addition, we repeated the Sanger sequencing using a new +ve strand primer that had been redesigned to have a mismatch with both pseudogene sequences at its 3′ end (Fig. 2D) and the c.5560G>A variant was still clearly visible (Supplementary Material, Fig. S2).
