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After incubation, single colonies were picked from the edge of the clearing zones caused by EMS.
Bacterial cells consume oil as a carbon source and thus show clearing zones around their colonies.
There was a clear difference between clearing zones diameters when bacterial cells cultures or their cell-free filtrates were used.
In agreement with previous studies, this study found clearing zones formation after 7 day with different bacteria consortia.
This study showed that a wasta consortium containing Bosea thiooxidans had the largest clearing zones and higher media viscosity reading.
Zones formed after incubation with free cells filtrate, suggest that these types of bacteria produce extracellular compounds that break oil and produce clearing zones.
Similar(27)
Clearing zone (mm) was determined after appropriate incubation time.
The size of clearing zone diameter of each isolate is depicted in Fig. 1.
Cellulase production was determined by CMC cup-plate clearing zone (CCZ) assay.
After incubation, the clearing zone diameter was measured.
Sedimentation was assessed by the occurrence of a clearing zone at the top of the culture.
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