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Hernández, who is recovering from surgery on his right foot, was cleared to remove his protective boot.
The resulting homogenate was cleared to remove all debris by centrifugation at 12,000g for 15 min at 4°C, the protein supernatant was collected and its protein concentration was determined by a modified Bradford assay [16].
Briefly, 1 ml of the culture supernatant was collected and cleared to remove any cells and cell debris by centrifugation at 1,000 g for 5 min, followed by filtration of the cleared supernatants through a 0.2 μm syringe filter.
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Rinse schoolbag in cold water several times, or until water runs clear, to remove excess dye.
Besides electrical wiring, there are other things that should be cleared away to remove fire risks.
The substrates cleaned with anionic and non-ionic surfactants were of particular interest as the application of surfactants is commonly followed by clearing steps to remove residue.
The elution was then cleared by centrifugation to remove any precipitated proteins and loaded onto the FPLC.
The refolding reaction mixture was then cleared by centrifugation to remove misfolded species.
Mr Silvester's suspension came as Ukip leader Nigel Farage said he had launched a clear-out to remove from the party anyone with "extremist, nasty or barmy views".
But they do show that even in states where the benefits are relatively high, there is a clear incentive to remove oneself quickly from the ranks of the unemployed.
Mr Farage claims has since launched a clear-out to remove from the party anyone with "extremist, nasty or barmy views", and he referred to this promise in his forecast, broadcast on the BBC's Sunday Politics programme, by saying there had been "outbreaks of sometimes barmy, occasionally extremist views from people of all political persuasions".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com