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In immunohistochemistry we found a clear staining pattern for SLC/CCL21 on podocytes and CCR7 on MC during nephrogenesis and in adult kidney.
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Cells were considered to be Cre positive only if they had a clear nuclear staining pattern co localizing with DAPI.
It was evident that the Gd3+ ProCA1-affi bound to the cell surface HER2 with a clear membrane staining pattern in AU565 cells at 4°C (Fig. 2A).
Consistent with the SEM and TEM data, cells expressing extracellular-domain-containing Podocalyxin mutants exhibited a clear punctate staining pattern on the apical surface of cells in protruding structures indicative of microvilli (figure 10A, B).
Unfortunately we were unable to observe a clear, punctate staining pattern with any of these antibodies indicative of endosome staining.
After two weeks in culture, E-cadherin showed a clear basolateral staining pattern regardless of whether MCF7 cells were grown alone or in co-culture with RMF.
However, in cells that were only HA-RANK-positive there was a clear membrane staining pattern and in cells that were only GFP-RANK-c-positive there was only cytoplasmic localization of the tagged protein.
MCT 1 showed a clear membranous staining pattern with substantial variation in intensity and extent; in some sections large MCT1 positive areas were seen, in other sections almost no MCT1 was present (fraction range 0.1 - 64%, median 14%).
Bleomycin-treated BI-1−/− mice had a much clearer collagen staining pattern than bleomycin-treated BI-1+/+ mice, suggesting an increase in fibrosis in the lungs of BI-1−/− mice.
Correspondingly, there is a clear filamentous tropomyosin staining pattern in the SH-EP cells.
We have demonstrated that antibodies against two different HER4 receptor antigen sites identify clear differences in staining patterns.
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