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The h100 bp construct has low but clear promoter activity (half of SV40) and is activated 3-4-fold by the zebrafish and SV40 enhancer.
Dual-strand clusters in contrast seem to be unique in flies: they lack a clear promoter (as defined by the lack of H3K4me2 peaks), 5′ methyl-guanosine caps, and clear transcription termination and seem to rather rely on Pol II read-through transcription from convergent neighboring gene pairs or noncanonical transcription initiation (Mohn et al., 2014).
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The traveling ratios given above were for all genes, whether they presented a clear promoter-proximal RNAPII peak or not.
We initially focused on genomic breaks in the transcribed region of the 5,140 genes containing a clear promoter-proximal peak.
Given that genes with a promoter-proximal peak are more likely to be active, we next focused on 5,140 genes with clear promoter-proximal peaks, and then asked how RECQL5 affects the traveling ratio in this group of genes.
First, RSC clears promoters by translating poly dA dT) into directional nucleosome removal.
MS-PCR proposes a clear MGMT promoter methylation status and divides the tumor samples into PCR-positive and –negative cases.
From these studies, a clear Plp promoter driven spatio-temporal patterning emerges for the CNS – that of neuronal inclusion early in postnatal development, with later stages defined by increased oligodendrocyte specificity.
It is not clear why promoter mutations were only found for a minority of the 39 DGC domain-containing proteins.
Breast cancer specimens exhibited strong ID4 mRNA downregulation (16-fold and 71-fold, respectively) in comparison to their corresponding normal tissues depending on clear ID4 promoter methylation.
Looked at through that lens, it's clear the promoters are simply repackaging an Indian tradition and marketing it as the new foam parties.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com