Sentence examples for clear insert from inspiring English sources
Following primary culture, cells were subsequently passaged in either monolayer culture (seeded at 5,000 cells/well of a Lab-TekR Chamber SlideTM (NapiervillervILle, IL) or into 3D culture in 1.2% Keltone LV alginate (Kelco, San Diego, CA) at a cell density of 50,000 cells seeded into the alginate which was then layered onto a Costar Transwell Clear Insert (Costar, Cambridge, MA).
Choroid plexus epithelial cells were isolated and cultured on Costar Transwell Clear inserts (0.4 µm pore size, 6.5 mm diameter) as previously described [41], [42].
Replicate collagen sponges were placed on Costar Transwell Clear Inserts (Costar) in 24-well plates and soaked with 2 ml MEM20.
Basal cells were then expanded into 75-cm flasks and upon confluence seeded at 10 cells/cm on 1.2-cm-diameter transwell clear inserts (Corning Incorporated) under BEGM for 2 days.
P.1 PBECs (60s) cultured on Transwell Clear inserts were found to give a maximum TEER of ∼1300 Ω cm (mean=789±18 Ω cm, n=91 inserts in 24 independent experiments and a minimum apparent permeability (Papp) to [C]sucrose of 3.0×10−6 cm/s (mean=6.07±0.32×10−6 cm/s, n=29 inserts in four independent experiments).
Replicate collagen sponges were placed on Costar Transwell Clear Inserts (Corning Incorporated-Life Sciences, Lowell, MA) in 24-well plates and fed three times per week with 2.0 ml of MSCBM with 10 ng/ml TGFβ (Cambrex Bio Science, Walkersville, MD) or without TGFβ (control).
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Cells were grown to confluence on Transwell-clear inserts (Costar).
The TEER of cell-free Transwell®-Clear inserts were subtracted from the obtained values.
Cells were grown in cell culture dishes or in Transwell-clear inserts (Costar, Corning).
Cells were used between passages 10 and 40 and seeded onto 1 (0.45 μm pores) or 4.5 (3 μm pores) cm Transwell-Clear inserts (Corning) at a density of 250 000 cells cm−2.
