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Circuits with combinatorial motifs yielded clear foci of synthetic PIP3 that can persist for nearly an hour.
This POF staining pattern was in agreement with a previous observation that POF signals decorating the pair of whole fourth chromosomes were seen as one or two clear foci in S2 and other somatic cells including ovarian follicle cells [36].
TssJ-mCh was not included in this analysis since clear foci could not be detected.
After repeated HAI (5×HAI) however clear foci of transfection of tumour vasculature and peri-tumour vasculature transfection were observed, estimated 2 3% positive cells.
In the FAS test, phalloidin labelled with FITC is used to detect this accumulation, using fluorescence microscopy [ 22]. Figure 6(a) shows clear foci of actin accumulation upon interaction of the starting EHEC EDL933 strain with HeLa cells.
Tam selected MCF10AT1 cells (Fig 1C), exhibited spindle shaped cells with clear foci formation indicating additional cellular transformation of the MCF10AT1 cell line consistent with the loss of contact inhibition, allowing the cell's ability for growth beyond confluency.
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