In order to assess the overall performance of the model collection, the individual hit lists from the FXR-actives, FXR-decoys, ChEMBL, DrugBank, and Virtual DB were merged for each model, respectively, and cleared from duplicate entries.
It is also possible that this duplication is unique to Atlantic salmon, since no clear evidence of duplicated elovl5 genes in other salmonids exist in the current sequence databases.
In addition, because Glp1r is only partially duplicated it is not clear that the extra copy is functional as discussed above.
Amplifications were performed in duplicate in clear 96-well plates on a LightCycler 480 II (Roche, San Francisco, CA) using manufacturer-recommended cycling conditions: 95°C for 10 min, 45 cycles of 95°C for 10 s, 60°C for 1 min with a ramping rate of 1.6°C/s, and a final melt-curve analysis.
It is clear that loss of duplicated genes is a very common fate after a genome duplication event but the present analysis nicely illustrates a late case of neofunctionalization. Our data suggest that basal teleosts and percomorphs kept two functional copies of AR whereas "intermediate" lineages such as Otophysi and Salmoniformes lost it secondarily (see below).
We analyzed WG duplicates and tandem duplicates because their duplication mechanism is clear and contrasting, large scale versus small scale, whereas dispersed and transposed duplicates can arise by multiple mechanisms.
All "coffee table books" were also part of the clear out, as were duplicates, foreign language material and items now available as an electronic resource.
The previously fabricated denture was duplicated in clear acrylic resin and used as a radiographic surgical guide using gutta-percha as radiopaque markers.
All ambiguous genotypes were also repeated in duplicates till clear genotypes were available.
Analysis of the PCA obtained from the seed samples showed clear differences between both duplicated lines and their respective donors.
