AdHuPA transduction resulted in clear-cut expression of human uPA protein detected by immunohistochemistry and induction of up-regulation in the expression of metalloproteinases MMP-3, MMP-9, and MMP-2.
Secondly, induction of differentiation experiments evidenced a clear-cut expression of glycophorin A (CD235a).
We observed clear-cut expression of Bax in 45.5% (10/22) of the evaluated specimens.
Importantly, the genes selected by this filtration on the basis of developmental expression profiles also exhibited a clear-cut expression pattern in testicular cells isolated from mice.
Moreover, a clear-cut expression of CCL21 was also observed in flat wall vessels of the superficial subintima of the sublining layer (Fig. 4c,d) [ 23].
In our study, both Hsp90 and Hsp73 exhibited a clear-cut expression in mitotic cells: Hsp90 seems to regulate the metaphase-anaphase transition [ 55], by controlling the stability of the centrosomal Polo kinase [ 56], whilst Hsp73 appears to be localized on the fibres of spindles and asters during metaphase [ 57].
When comparing the BMP expression profiles of the different types of mammary tumor clones, we were not able to see any clear cut differences in either BMP expression repertoire or expression levels when comparing clones from the spindle cell and osteosarcoma.
In line, we found no clear cut decrease in the local expression of IL-1β and IL-6, even though these are produced by B cells, T cells and macrophages.
A clear cut difference, interpreted as diminished expression of Melan-A or HLA-class I, was considered starting from a change of 50% or more in the comparison of the sections prior to and after vaccination, as illustrated in Figure 5.
The bridging of this work from chickens (where the correlation data between peptide binding promiscuity and expression levels are clear cut) to humans is a major point of the paper, so the expression data need to be definitive.
Overall, these array data reveal a profile consistent with a less aggressive, and more favorable, prognostic profile for MDA-MB-231 cells treated with LHB589, while the less biologically sensitive MDA-MB-468 and MCF-7 cell lines display a less clear cut picture for panobinostat-induced gene expression in cells of the basal-A and luminal subtypes.
