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Everything is wonderfully spiced, providing not merely heat but a clear amplification of the flavors.
An experiment for observing a clear amplification must be designed through the RMHD simulations so that the RMI takes place in the low-density area between two targets.
Nested PCR reactions were then carried out to obtain clear amplification bands that served to construct SSU rDNA libraries (Table 1).
Reactions that showed clear amplification bands were sequenced at the Genome Quebec Innovation Center (Montreal, Qc), using the specific primers (Table 1).
Clear amplification products were obtained for 58 primer pairs.
All 10 microsatellites showed a clear amplification pattern after capillary electrophoresis.
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Of the 192 SNP primers evaluated, 179 (93%) produced clear amplifications.
All the strains of Brucella spp and M. tuberculosis complex showed clear DNA amplification, confirmed by melting curve analysis and sequencing the amplified products, which perfectly matched the 207 bp and 164 bp fragments corresponding to B. abortus and the SenX3-RegX3 interegion regiof of M. tuberculosis complex.
RT-PCRs with universal flavivirus primers resulted in clear PCR amplification products of the expected lengths.
Thirty-two loci exhibited clear PCR amplification with fragment sizes ranging from 50 to 500 bp (Table 1).
After the initial double-repeated primer test screening using our protocol, 19 markers were selected out of the initial 22 markers set, for their clear reproducible amplification pattern.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com