Sentence examples for cleanup plate from inspiring English sources
Oligonucleotides were removed from the PCR mix by PCR 96 cleanup plate (Millipore).
In order to remove unincorporated dNTPs and primers during the amplification reaction, the positive amplicons were purified through MultiScreen384PCR Cleanup plate (Millipore, Carrigtwohill, Co. Cork, Ireland).
Amplicons were detected on a 1.5% ethidium bromide agarose gel and purified by using a UniFilter Multiscreen PCR cleanup plate (Whatman, Brentford, UK) according to the manufacturer's instructions.
RNA was reverse-transcribed according to the Omniscript RT™ kit (Qiagen, Valencia, CA) instructions and subsequently purified using the Montage PCR 96-well cleanup plate (Millipore, Billerica, MA).
The multiplexed PCR products were diluted to 50 μl with sterile water and purified by ultra-filtration to remove excess PCR primer and dNTP using a Montage 384 PCR cleanup plate (Millipore) according to the manufacturer's instructions.
The sequencing reactions were performed using a 2 min initial denaturation step, followed by 25 cycles at 96°C for 10 sec, 50°C for 5 sec and 60°C for 4 min and then purified from unincorporated primer and BigDye excess through Multiscreen384SEQ Sequencing reaction Cleanup Plate (Millipore, Carrigtwohill, Co. Cork, Ireland).
PCR products were purified with a MultiScreen Resist vacuum manifold and PCR96 Cleanup Plates (Millipore).
PCR product was cleaned with Millipore PCR 96 cleanup plates (Millipore, Billerica, MA).
PCR products were purified using MultiScreen PCR μ96 cleanup filter plates from Millipore.
PCR products were purified with PCR cleanup filter plates (Millipore) and sequenced using M13 primers and BigDye v.1.1 on a 3730 DNA analyzer (Applied Biosystems).
The PCR cycling conditions were 95°C for 5 min, 35 cycles of 30 s at 95°C, 45 s at 55°C, and 90 s at 72°C, followed by a final elongation step of 72°C for 2 min. The PCR products of the six animals from the discovery panel were purified using millipore PCR cleanup filter plates (MSNU03050) and sequenced using the DETT sequencing kit according to the manufacturer's specifications (GE Healthcare).
