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The cleaned products were shrink-wrapped and put in master cartons to look as if they had been bought from the manufacturer, then sold to corrupt wholesalers who would commingle the stolen goods with legitimately purchased products and sell them back to retailers — often to the same store from which they had been stolen.
Cleaned products sequenced by Macrogen inc.
Cleaned products were cloned into pGEM-T Easy vectors (Promega), and transformed into E. coli JM109 competent cells (Promega) following the manufacturer's protocol, except that all reactions were halved.
The cleaned products were directly cycle sequenced in the forward direction using the L4447 primer.
The cleaned products were run on gels, to verify the presence of the expected bands.
The cleaned products were cycle sequenced using a big dye-terminator sequencing kit (Applied Biosystems, Foster City, CA).
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The use of ion mobility thus provided a simplified and cleaned product ion spectrum that facilitated the identification process.
Enriched, cleaned product was diluted to 5 pM and submitted for sequencing on an Illumina GAII sequencer at the University of British Columbia (Vancouver).
Enriched, cleaned product was diluted to 5 pM and submitted for sequencing on an Illumina GAII sequencer at the Center for Genome Research and Biocomputing (CGRB) at Oregon State University (Corvallis, OR, USA).
Cleaned product was transferred to 384 well plates, dried in a speedvac and resuspended in 3 × SSC and 1.5 M Betaine buffer for printing.
Method's dedication to clean products and production -- 75percentt of its product line is Cradle to Cradle Certified -- guarantees a healthful work place for employees, a lovely public space for Pullman, and safe, delightful products for its customers.
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