Body weights were recorded and kidneys dissected, cleaned of fat and blood vessels, decapsulated and weighed.
The mesenteric bed was removed, pinned out on a silicon based Petri dish before being cleaned of fat and connective tissue.
The heart lung block was pinned out in a dissecting dish containing PSS, to allow the descending thoracic aorta to be carefully cleaned of fat and connective tissue.
Uterine horns from mice on day 4 of pregnancy were cleaned of fat tissues, slit longitudinally, and washed thoroughly in Hanks' balanced salt solution (HBSS, Sigma) without Ca2+/Mg2+ and phenol red.
Tumors and organs were dissected, cleaned of fat and connective tissue and weighed.
After the tissue collection, the colonic segment was cleaned of fat and mesentery and blotted on filter paper.
After removal, the ovaries were cleaned of fat, snap frozen in liquid nitrogen, and stored at -70°C until the various assays were performed.
The ileum was immediately removed, cleaned of fat and connective tissue, immersed in physiological solution at room temperature and bubbled with carbogen mixture.
Following euthanasia, whole ureters from rat of either sex were dissected and cleaned of fat and connective tissue, cut into small segments (4 5 mm long) and placed in normal Krebs solution until further use.
Tail was removed from rat humanely killed by cervical dislocation under CO2 anesthesia in accordance with Home Office legislation Artery was dissected from the ventral grove, cleaned of fat and kept in physiological saline before use.
