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Bone marrow samples from wild-type Sp1cko/cko::Sp3cko/wt mice and from LysM-cre::Sp1cko/cko::Sp3cko/wt::YFP/wt mice were subjected to clean up using a MACS dead cell removal kit (Miltenyi Biotec).
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In a final step, embedded coordinates are cleaned up using a crude force field and the bound matrix [23].
The toluene extracts for the EROD assay were cleaned up using a multi-layer silica column in order to isolate the persistent lipophilic compounds according to the protocol shown in[58] and[59].
The resulting PCR product was cleaned up using a QIAGEN Minelute PCR purification kit and then ligated using a ZeroBlunt TOPO kit (Invitrogen) into linearized pCR4 Blunt-TOPO vector.
The iTRAQ-labeled samples were pooled together, cleaned up using a cation exchange column, separated by reversed-phase high performance liquid chromatography and analyzed by ABI 4800 MALDI TOF/TOF MS/MS.
The sample was cleaned up using a Zymo kit-5 (using 700 μl binding buffer).
The peptides were cleaned up using a Sep-Pak C18 cartridge as described above.
Primary PCR products were cleaned up using a Qiaquick Gel Extraction Kit (QIAGEN) column.
The reaction was cleaned up using a Qiaquick MiniElute column (Qiagen), eluting the cDNA in 20 μl EB.
Prior to submission to the automated reaction to Eurofins MWG Operon, amplified products were cleaned up using a NucleoSpin Extract II kit (Macherey-Nagel, Düren, Germany).
Labelled DNA was cleaned up using a microspin G50 column (Amersham), boiled for 5 min, then added to the prehybridization buffer and left to hybridize overnight at 65°C.
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