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PCR products were cleaned with DNA Advanced™ DNA Clean Up System (Viogene BioTek Corp., Sijhih, Taiwan) following the manufacturer's protocol.

Amplified products were purified with the Wizard MagneSil PCR Clean Up System (Promega, Madison, WI).

In addition, hot gas clean-up system will be examined in above 2 gasifiers.

DNA purification was performed using Wizard SV Gel and PCR Clean-Up System (Promega).

After gel purification by the Wizard® SV Gel and PCR Clean-Up System (Promega), PCR products were directly sequenced.

Products to be sequenced were previously purified using Wizard SV Gel and PCR Clean-Up System (Promega).

Modified DNA was purified using the Wizard DNA clean-up system (Promega, Madison, WI, USA).

The two resulting PCR combined products were then purified using the Wizard PCR clean-up system (Promega, Wisconsin, USA), resuspended in 50 µl of milliQ water supplied with the clean-up system and then quantified spectrofluorimetrically as described above.

After the DNA sample was purified with a sepharose column (Wizard DNA Clean-Up System; Promega, Madison, WI).