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Thereafter, PCR product was cleaned up using DNA, RNA and protein purification REF 740609.250 Gel and PCR clean up protocol (January 2012/Rev.02).
The procedure was used to measure spirolides in mussel samples using an extraction and clean up protocol suitable for the FP assay.
Total RNA was recovered from the gDNA elimination reaction using the RNeasy Micro kit (Qiagen) RNA clean up protocol.
RNA was extracted by a Trizol chloroform method before using the RNeasy clean up protocol from Qiagen.
For macroarray analysis, RNA was treated with RNase-free DNaseI (QIAGEN, Germany) in order to eliminate contaminant DNA and purified using the RNeasy Mini Kit (QIAGEN, Germany), according to the RNA clean up protocol.
The clean up protocol was fully automated using a Magnatrix 1200 biomagnetic workstation (NorDiag AB, Hägersten, Sweden).
Similar(52)
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