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The transcripts were purified using the RNeasy clean up kit (Qiagen).
Mutant library thus obtained was further purified using QIAGEN PCR clean up kit and used for further transformation.
cDNAs larger then ∼70 nt were purified using MinElute (Enzymatic Clean up Kit) filtration columns (Qiagen).
Synthesized aRNA was purified with the E.Z.N.A. MicroElute RNA Clean Up Kit (Omega BiOmega).
The labelled aRNA was purified with the E.Z.N.A. MicroElute RNA Clean Up Kit.
After fractionation, samples were desalted using the 2-D Clean Up kit.
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Pooled volumes of the library were purified using the QIAquick MinElute Reaction Clean-up Kit.
The plasmid with T7 promoter was linearized by restriction enzyme Sac-I and then purified using DNA clean-up kit.
The sample was cleaned using 2D-clean up kit.
The second desalting procedure uses a 2D-clean up kit (Figure 1D F).
Processed DNA samples were purified using the PCR-clean up kit (Promega).
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