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Clean tags mapping to reference sequences from multiple genes were filtered out, and the remaining clean tags were designated as unambiguous clean tags.
All clean tags were mapped to reference sequences using bowtie software and allowing 2-bp mismatch.
For gene expression analysis, the number of unambiguous clean tags for each gene was calculated and normalized to TPM (number of transcripts per million clean tags).
The clean tags were designated as unambiguous clean tags.
Remainder clean tags were designed as unambiguous clean tags.
The remaining clean tags were designated as unambiguous clean tags.
The remainder of the clean tags were designed as distinct clean tags.
Clean tags mapped to reference sequences from multiple genes were filtered, and the remaining clean tags were designated as unambiguous clean tags.
After filtering out clean tags mapping to reference sequences from multiple genes, the remaining clean tags were designated as unambiguous clean tags.
99.5% of raw tags in each library were clean tags.
Length distribution of clean tags is then summarized.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com