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In the presence of GO (lanes I III), a clean gel was observed without EB-derived background noise.
Each PCR product was mixed with one volume of denaturating solution (95% formamide, 20 m M EDTA, 0.05% xylene cyanol, 0.05% bromophenol blue), heated for 5 min at 95°C, cooled on ice and subjected to nondenaturing electrophoresis in a 10% PAGE (Clean gel 48S kit, Amersham Pharmacia Biotech, Sweden).
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Surfactant-based fracturing gels are considered as clean gels due to the absence of insoluble residues in their composition.
Due to my years of scientific training at Sichuan University, I quickly produced clean gels and reliable results.
Cleaned gel was dried at 40°C for 24 hours and stored in a plastic container at room temperature.
Reaction products were purified directly (MinElute PCR Purification Kit; QIAGEN, Valencia, CA, USA) or excised from the gel and cleaned (Gel MinElute PCR Purification Kit; QIAGEN).
The electrode sites were cleaned using Neoprep cleaning gel.
Amplified PCR products were cleaned up by the PCR clean-up Gel extraction Kit NucleoSpin® Extract II (Macherey-Nagel, Dueren, Germany).
PCR-products were cleaned using gel electrophoresis and the QIAquick Gel Extraction kit (Qiagen).
Libraries were cleaned and gel purified, excising DNA ∼250 850 bp (250 1250 bp for the E. coli samples) in a triangle shape as above, diluted to 10 nM, and sequenced on the Paired-end module of the Genome Analyzer II following Illumina protocols for 40×80 bp reads.
A clean plastic gel-loading tip was then lightly dragged across the bilayer cup orifice to paint a lipid bilayer over the micropore.
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CEO of Professional Science Editing for Scientists @ prosciediting.com