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For HEK-293 cells, the above protocol was modified to obtain clean fractions.
Clean fractions were collected and concentrated to ∼100 μM for further use.
Clean fractions were collected and concentrated to ∼100 μM for further use, and the final protein concentration was determined spectrophotometrically.
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The cleaned fractions were then lyophilized again and stored at −20°C until analyzed by mass spectrometry.
Cleanest fractions were pooled, adjusted to a 50% saturation of ammonium sulfate and mixed at 4 °C for 20 min.
We analyzed the cleaned fractions using a Varian CP-3800 gas chromatograph (Varian, Inc., Walnut Creek, CA) equipped with a Ni electron capture detector, a CP-8200 autoStarler, a Star Chromatography Workstation (version 5; Varian Inc ., and an SPB-608 fused silica capillary column (30 mm × 0.25 mm × 0.25 μm film thickness; Supelco, Bellefonte, PA).
A simple correlation has been proposed to determine the cleaned fraction from pressure differences as a measure of the efficiency of cleaning.
After 50 μL of the syringe spike solution ([C12]PCB15 for evaluation of recovery yields of monochlorinated to pentachlorinated biphenyl surrogates, and [C12]PCB170 for hexachlorinated to decachlorinated biphenyl surrogates) was added to the cleaned fraction obtained by each method, the solution volume was reduced to 0.2 mL by means of a rotary evaporator and under a nitrogen gas stream.
The purpose of this study was to fractionate switchgrass (SG) to obtain hemicellulose-, lignin-rich frandions and highly digestible pulp, using a clean fractionation (CF) approach.
Clear fractions.
The overall process can generate an ultra-clean JP-8 light fraction fuel with approximately 300 ppb sulfur residual.
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