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A specific PCR product corresponding to the excised lox cassette could be amplified in 17 out of 19 femaleale individuals; sequencing the PCR products confirmed clean cassette excision, leaving a single remaining loxP site (data not shown).
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Clean the gear cassette on the rear wheel carefully.
Using equipment he bought from the government, he started his own company in the early 1980s, first making a liquid used to clean the heads of cassette tape players, then an underarm deodorant.
These moving live takes are impressively cleaned up from a cassette recording made at Covent Garden's Canteen five years earlier, with Baker accompanied by a supple and sensitive local trio led by elegant John Dankworth pianist John Horler.
In the third step, I-SceI-mediated DSB resulted in 27/36 (75%) of the colonies showing precise removal of the cassette, resulting in a clean insertion [Figure 3B (c)].
Removing the sacB-kan cassette from the chromosome, leaving a clean deletion: Day 1: Transform the exchange plasmid (containing the flanking regions and no sacB-kan cassette) into the intermediate strain with the sacB-kan cassette in the chromosome Plate 250 μl of the recovered transformation onto LB (Luria-Bertani) agar plates containing chloramphenicol (25 μ/ml).
Two tracks, "Do It Clean" and "Read It in Books", were included on the cassette but initially omitted from the LP version of the album because the managing director of Warner Bros., Rob Dickins, thought that they contained obscenities.
The results of D.J. Screw's labors often sound like rap records played underwater on an old cassette deck that's running out of batteries and needs its tape heads cleaned.
… The young woman cleaned the ashtrays and bottles and went back to the counter to insert a tape cassette, a medley of stuff in English: Elton John, Gilbert and Sullivan, the Carpenters, the Seventies, the Eighties.
Some of the earliest footage captures ordinary life with long, static shots: a mosque being cleaned at dawn, a metalworker chiselling out a repetitive pattern, an argument over sound quality at a cassette shop.
Cassettes (Figure 4) are designed to contain all disposable components and fluid paths, eliminating the need to clean or customize the apparatus between syntheses.
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