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Note that the detection thresholds used here are not optimized with respect to the number of concordant classifications of probes.
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To test for this, we inspected spot intensities and performed a more finely-grained classification of probes.
Additional file 4 shows the degree of concordance between the platforms with respect to the classification of probes as amplifications, deletions or normal.
It is clear from the GACK classification of probe absences (see Additional Files 4 and 5) that SCO6832 is largely intact/present, as of the 18 probes targeting this gene all but three are present/non-divergent, i.e. only the first probe targeting the 5' terminus and the two probes targeting the 3' terminus do not bind.
It is also extremely flexible, in that a classification for any gene can be generated by aggregating the classifications of the probes targeting that gene.
Additional file 5: Table S3 (Online Resource 3) describes the classification of all probes used with their flanking sequences, SNP and physical position.
Functional classification of select probe sets was performed at NIH DAVID server (http://apps1.niaid.nih.gov/david/upload.asp).asp
This emotional reaction is task-irrelevant because correct response selection requires consideration only of the cognitive classification of the probe as having positive or negative content (and in integrating this information with cue classification).
In like manner, using CFR for the 60 month classification experiment, the number of probes was reduced from 22,282 to 829 using the same hard cut-off t-test p-value of 0.05.
This classification method uses a set of probes comprising short, highly conserved amino acid sequences.
SY and WX performed the computational anlaysis involved in the electronic masking of probes, functional classification and over-representative analysis.
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