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The 1601 tissue-selective genes covered a wide range of cellular and molecular functions as they could be annotated into 1694 distinct Biological Process, 1094 Molecular Function and 290 Cellular Component functional families from the three gene ontology classifications (File S1, tables 0.2, 0.3 and 0.4).
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We recommend having the classification file open to explore while reading the descriptions and instructions below [see Additional file 1].
Total 29,894 sequences were assigned to COG classifications (Additional file 2: Table S4, Additional file 1: Figure S3d).
This underlines the ambiguity of old classifications (Additional file 1 - Table S1).
We confirmed that there was no significant difference between the classifications (Additional File 19).
These piRNAQuest piRNAs were then used for further classifications (Additional file 2: Figure S1).
In 309/321 (96.3%) isolates, KvarQ detected MTBC lineage-specific SNPs that were in agreement with the previous classifications (Additional file 2).
In order to see which parts of metabolism are more enriched with essential genes, we classified reactions into 8 functional pathway classifications (Additional file 1: Table S10).
Significant differentially expressed cardiac transcripts in TFA + MSG-fed versus TFA-fed mice were examined for enrichment of GO and KEGG classifications (Additional File 8, Table S8).
We analyzed the correlation between the topology and biological function of the highly expressed genes using the GO classifications (Additional file 5: Figure S2).
In total, 5,491 of the 18,624 sequences retrieving a hit from the NCBI Nr database could be assigned to COG classifications (Additional file 7).
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