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The RBE for the sample was calculated as the fraction methylated/ methylated + unmethylated) ignoring indeterminate classification reads.
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Also results on de novo assembly and metagenomic reads classification show that the introduction of quality values improves over standard alignment-free measures.
Fig. 3 Taxonomic classification of OTUs at phylum level (JM1 Jakrem samples, YM1 Yumthang samples) Fig. 4 Taxonomic classification of reads at phylum level (JM1 Jakrem samples, YM1 Yumthang samples).
We therefore utilized Kraken for taxonomic classification of reads for all subsequent analyses.
We found similar concordance between RDP and STIRRUPS classification with reads classified to other genera.
22 For taxonomic classification, trimmed reads were blasted against the SILVA 16S specific database (V.110).
Taxonomic classification of reads was then undertaken using the classify function of guppy, a part of the pplacer package.
After classification, redundant reads from each class were eliminated as described above, and methylation extractor was run for each class as well as for all reads combined.
Composition of the biogas-producing microbial community was obtained by taxonomic classification of reads base on the M5NR database on the MG-RAST platform.
Subsequently, the taxonomic classification of reads was also made with less stringent setting (Min Support: 1, Min Score: 10, Min Score/Length: 0.00, Top Percent 100).
After classification, short reads mapped to a specific gene were collected in order to assemble them into contigs for further analysis.
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