Following our PCA and classification of the accessions merging all the parameters measured in both watered and drought conditions, several accessions appear to be good candidates for further studies which may reveal unknown major genes and alleles for responses to drought.
The classifications of some accessions that exhibited characteristics that obviously contradicted their documented type were corrected, and accessions with no available information in published material were classified based on the following parameters: presence of flowers on the main stem, number of seeds per pod, pod appearance and shape, total number of branches, and plant growth type.
Classification of the various accessions used in this study is schematized in figure 3. 40024 genomic DNA was extracted using DNeasy ®Plant Mini Kit (Qiagen, Courtaboeuf, France) following the manufacturer's recommendations and the other DNAs were a generous gift from Dr F. Pelsy (INRA, Colmar, France).
In a first round of classification, all accessions were compared against the cDNA of the CDP04928 accession, and 36 different bands were detected.
One of the other notable exceptions to the correspondence between genetic and passport classification was the subgroup comprised of accessions that were listed as SLL vintage, but instead were genetically classified as a SLC group closely related to SLC Ecuador.
To achieve this goal, an integrated approach involving the monitoring of double-ridge (DR) formation and final leaf number (FLN) was used to verify the growth habit classification of seven diploid Brachypodium accessions.
Systematic classification of terminals and GenBank accession numbers for sequences and collection information for newly sequenced species and their accession numbers in the CT-UFMG (Coleção taxonômica da Universidade Federal de Minas Gerais) and MZSP (Museu de Zoologia da Universidade de São Paulo).
In the orchid genomes, ~ 400 and ~ 300 RLKs have been detected in Dendrobium and Phalaenopsis respectively, accounting for 1.4 and 1% of the respective proteomes (containing 29,400 and 29,679 annotated proteins) (see Table 2 for the complete list of accessions and their classification).
The raw reads of 48 O. rufipogon accessions were obtained from NCBI SRA under project accession numbers listed in Supplementary Data 2. The metadata associated with the subgroup classification of these 48 O. rufipogon accessions was extracted from prior studies10,30.
