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The effectiveness of the proposed method is validated on a publicly available flower classification database with promising results.
A protein structure classification database search indicated that BoNT and NTNHA share a similar domain architecture, comprising a zinc-dependent metalloproteinase-like, BoNT coiled-coil motif and concanavalin A-like domains.
Like DALI and SSAP, CE has been used to construct an all-to-all fold classification database from the known protein structures in the PDB.
SCOP provides a manually curated protein classification database of domains defined largely by homology.
The structure-based approach uses protein structure data and allows searching for similar proteins over a structure classification database using structure alignment methods.
We employed the structure alignment program SSM [20] and carried out researches on 75 protein families from the SCOP 1.73 structural classification database [21], [22] (Table S1).
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For transporters, a more detailed analyses was performed with the Transporter Classification Database TCDB database [ 151].
It has been applied in an all-to-all fashion to produce a hierarchical fold classification scheme known as CATH (Class, Architecture, Topology, Homology), which has been used to construct the CATH Protein Structure Classification database.
A BLAST analysis (blastx, e-value<1e-10) of the unique set was conducted against the Transport Classification Database (TCDB) (http://www.tcdb.org/) and the genes matching sequences from that database were again manually reviewed and assigned to a category from the Transport Classification System [57].
Genes encoding transporters were annotated by BLASTP using transporter encoding genes retrieved from the Transport Classification database with a cut-off of Evalue1e-20.
Information on transporters transferring metabolites between the different compartments as well as between the host and the parasite, were mostly obtained from the MPMP website, and in addition, from the Transport Classification database http://www.tcdb.org/, BioCyc, and Reactome database.
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