In the field of proteomics, the technology avoids tedious and time-consuming extraction and fractionation steps classically required for sample analysis.
Accredited laboratories, able to deliver certified results, performed the final quality control tests such as determination of pH, pyrogenicity and sterility, classically required in clinical studies.
In the single-SNP analysis and considering a general codominant model for each SNP effect, one SNP, rs512625, showed association with psoriasis that reached the 1% significance level classically required for replication (Table 2).
To achieve some of the echocardiographic images in cattle, physical strength is classically required [ 17].
Eukaryotic transcription is a complex mechanism classically requiring core promoter factors and gene-specific transcription factors, which assemble cooperatively on cognate DNA elements located on the promoter, upstream of the gene coding region, marked by the ATG "start" codon [ 14].
Even though caspase-independent mechanisms mediated by the apoptosis-inducing factor (AIF) have been described, the activation of caspases is classically required to initiate the process of apoptosis [ 32].
SLiMs are classically required to make interactions with structured globular domains in trans (Stein and Aloy, 2008), but intramolecular contacts following post-translational modifications have also been reported in a few cases (Pawson et al., 2001).
This reaction classically requires a redox active metal (M) to propagate the following reactions: 1 2To function as a catalyst, Cu II -Ybt must be regenerated, not Cu II -Ybtduring superoxide exposure.
Muscle atrophy occurring via inflammatory cytokines classically requires activation of the transcription factor NF-κB (nuclear factor kappa light chain enhancer of activated B cells) [ 52– 54], which in turn can increase muscle protein degradation, leading to rapid limb and respiratory muscle myofiber atrophy.
Indeed, in our cohort of surgical specimen, 53 (46.5%) of our routine consecutive patients presented a percentage of tumour cells under the value (50%) classically required for direct sequencing; and in addition, some patients with a percentage of tumour cells sufficient for direct sequencing, appeared to have K-Ras mutations at a low frequency.
