Sentence examples for classical transcript from inspiring English sources

However, for less well studied fruits, transcriptome analyses are based on 'home-made' microarrays, such as the μPEACH1.0 array [ 6], or classical transcript profiling by Differential Display-PCR or cDNA-AFLP [ 7- 9].

Two splice variants have been described for the proto-oncogene MYCN, the classical transcript that consists of three exons and a shortened ΔMYCN transcript that lacks exon 2. ΔMYCN is expressed in several fetal tissues, but its expression has not been reported in NBs.

For instance, classical complement component transcripts increased in head kidney while only alternative pathway transcripts increased in pyloric ceca, different β-defensins increased in head kidney but remained constant in pyloric ceca.

Each cell type is defined largely by the pattern of expression of the classical prestalk specific transcripts ecmA and ecmB (Jermyn et al., 1989; Williams et al., 1989; Jermyn and Williams, 1991; Early et al., 1993), and by their position in the migratory slug and final fruiting body.

In agreement with these results, we observed low levels of mRNA transcripts of classical HLA-I molecules (HLA-A and -B) and lack of the non-classical molecules HLA-E, -F and –G in both cell lines (Figure 1B).

Similar to higher plants and other red algae, classical caspase genes or transcripts were not found in Py. haitanensis.

Finally, to confirm the importance of Th1-dominant responses in lupus-like pathogenesis, mRNA transcripts of classical Th1 and Th2 cytokines (IFN-γ and IL-4, respectively) in the lungs and lymph nodes were also determined (Fig. 8).

Beside the classical approaches for profiling transcripts like Northern blots, reverse-transcriptase (q PCR, RACE (rapid amplification of cDNA ends), and microarrays, the recent development of RNAseq has revolutionized transcriptomics.

Suzuki et al.[ 9] described that transcripts encoding classical HSPs accumulated to a similar level in WT and transgenic plants over-expressing MBF1c; they suggested that the enhanced tolerance of these plants to osmotic and heat-shock stress was associated with the expression of other stress-responsive genes rather than with the constitutive expression of HSPs.

To determine a relative order of potency, we first examined the transcript levels of classical PXR, VDR and FXR target genes in response to 48 hours exposure to either LCA or PCN: For this purpose CYP3A1, GSTA2 and ABCC2 represent PXR target genes [23], CYP24 and KLK6 represent VDR target genes [25], while Fibrinogen β and UGT2B4 represent FXR-target genes [24].

Since the absence of Xrn1 leads to the accumulation of decapped RNAs (Hsu and Stevens, 1993), we could not use the TSS sequencing methodology applied for the other mutants and we thus used, for this particular experiment, a classical RNA sequencing approach for transcript quantification (see 'Materials and methods').