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The plaque assay is essential for virion quantitation but the classic protocol requires considerable efforts.
BAL was performed using a classic protocol.
Thus, we examined cell wall proteins released by enzymatic treatment (lysozyme+mutanolysin) or by hot acid, a classic protocol for extraction of M protein [33].
In humans, it has recently become possible to approximate this classic protocol non-invasively by combining transcranial magnetic stimulation (TMS) with hd-EEG [12]: high-frequency electrical stimulation can be safely substituted by repetitive TMS (rTMS), while changes in cortical responses to test TMS pulses can be assessed with hd-EEG.
The equation study used the classic protocol.
For β-actin IF, a classic protocol was used.
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Finally, MCOR algorithm is verified by simulation using nsclick software and compared its performance with the classic protocols: ExOR, TAODV and Watchdog-DSR.
Considering precognition as elusive and assuming that people should present random and precognition patterns when performing the classic protocols of the area, we proposed to apply the change point method to deal with this, conjointly searching for a rigorous cut-off value for identifying precognition.
Methods and Results: We drew from classic protocols for library enrichment by digestion, ligation, amplification, hybridization, cloning, and sequencing.
There are currently a number of classic protocols to achieve dehydration based on either simple air drying, vapor diffusion or soaking with dehydrating salts and/or precipitants [98,99,101].
To isolate mitochondrial complexes, we have combined elements from the classic Laemmli protocol and blue native polyacrylamide gel electrophoresis (BN PAGE) methods to develop a straightforward modified native electrophoresis protocol.
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